MALDI Imaging to verify tissue distribution of a new agents in magnetic resonance imaging.

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Düwel, S. et al. (2017). Imaging of pH in vivo using hyperpolarized 13C-labelled zymonic acid, Nature Communications, doi: 10.1038/ncomms15126.

By precisely and quantitatively recording the histological distribution of MRI agents, we can make a decisive contribution to the further development and improvement of these substances.

Proof that MALDI mass spectrometry imaging can be used on formalin-fixed and paraffin-embedded tissues

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Ly, A. & Buck, A. et al. (2016). High Mass Resolution MALDI Mass Spectrometry Imaging of Metabolites from Formalin-Fixed Paraffin Embedded Tissue, Nature Protocols, DOI: 10.1038/nprot.2016.081

The assessment of metabolites in formalin-fixed and paraffin-embedded (FFPE) tissue samples has been met with concern due to the potential effects of tissue processing on metabolite content and chemical state. This publication presents a protocol for the in situ analysis of metabolites from FFPE samples and proves that MALDI-FT-ICR mass spectrometry imaging platforms can be applied on FFPE tissues. It is demonstrated, that the metabolite content is largely conserved in FFPE tissue samples.

First analytical proof for in situ imaging of metabolites from formalin‐fixed, paraffin‐embedded (FFPE) human tissue samples

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Buck, A. & Ly, A. et al. (2015). High-resolution MALDI-FT-ICR MS Imaging for the analysis of metabolites from formalin-fixed paraffin-embedded clinical tissue samples, The Journal of Pathology, doi:10.1002/path.4560

Clinical tissue samples are commonly conserved as formalin‐fixed, paraffin‐embedded (FFPE) patient samples and can be archived for many years. In this study, MALDI-FT-ICR mass spectrometry imaging was used to analyse the metabolite landscape in FFPE tissues. The localization of different metabolites were demonstrated and used to answer tumour biology and clinical questions.