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An unparalleled approach for MALDI mass spectrometry imaging of metabolites in FFPE tissue

Formalin-fixed, paraffin-embedded (FFPE) tissue samples possess great potential to provide metabolic insights into physiologically normal, predisposed and pathological processes. Building on established protocols and applications expertise in mass spectrometry imaging of metabolites in FFPE tissues, multiple biomolecules can be analyzed label-free with high spatial resolution in the context of tissue morphology. Theratype’s molecular imaging approach […]

SPACiAL: A new method for immunophenotype-guided imaging mass spectrometry

Prade, V. M. and Kunzke, T. et al. (2020). De novo discovery of metabolic heterogeneity with immunophenotype-guided imaging mass spectrometry. Molecular Metabolism, DOI: 10.1016/j.molmet.2020.01.017. A new method for immunophenotype-guided imaging mass spectrometry allows accurate, objective and time-efficient annotation of specific cell types within a tissue section. This automatic pipeline represents an important step towards the […]

Mitochondrial dysfunction has a substantial influence on amino acid metabolism in cachectic skeletal muscles

Kunzke, T. et al. (2019). Derangements of amino acids in cachectic skeletal muscle are caused by mitochondrial dysfunction. J Cachexia Sarcopenia Muscle, doi: 10.1002/jcsm.12498 . Cachexia is a major cause for cancer-associated deaths. This study investigates the amino acid metabolism in cachectic muscles with in situ-omics techniques. Concentrations of amino acids, proteins, as well as […]

Proof that MALDI mass spectrometry imaging can be used on formalin-fixed and paraffin-embedded tissues

Ly, A. & Buck, A. et al. (2016). High Mass Resolution MALDI Mass Spectrometry Imaging of Metabolites from Formalin-Fixed Paraffin Embedded Tissue, Nature Protocols, DOI: 10.1038/nprot.2016.081 The assessment of metabolites in formalin-fixed and paraffin-embedded (FFPE) tissue samples has been met with concern due to the potential effects of tissue processing on metabolite content and chemical […]

First analytical proof for in situ imaging of metabolites from formalin‐fixed, paraffin‐embedded (FFPE) human tissue samples

Buck, A. & Ly, A. et al. (2015). High-resolution MALDI-FT-ICR MS Imaging for the analysis of metabolites from formalin-fixed paraffin-embedded clinical tissue samples, The Journal of Pathology, doi:10.1002/path.4560 Clinical tissue samples are commonly conserved as formalin‐fixed, paraffin‐embedded (FFPE) patient samples and can be archived for many years. In this study, MALDI-FT-ICR mass spectrometry imaging was […]