Entries by Achim Buck

A new multimodal procedure combining MALDI imaging with imaging microscopy for improved therapy prediction.

Huber, K. et al. (2019). Multimodal analysis of formalin-fixed and paraffin-embedded tissue by MALDI imaging and fluorescence in situ hybridization for combined genetic and metabolic analysis, Laboratory Investigation, doi: 10.1038/s41374-019-0268-z. The study presents a multimodal approach for the identication of genetic and metabolic properties from one tissue section. Combined with clinical data, including survival and […]

Imaging analysis can unravel the novel role of D-serine in regulating systemic glucose metabolism through modulating insulin secretion

Suwandhi, L. et al. (2018). Chronic d-serine supplementation impairs insulin secretion, Molecular Metabolism, doi: 10.1016/j.molmet.2018.07.002. Mass spectrometry is suitable for the visualization of metabolically important neurotransmitters in tissues and can be used in studies that include the evaluation of imbalances in neurotransmitter levels.

Pulmonary fibrosis: Investigating the metabolic processes of the antifibrotic agent pirfenidone.

Sun N et al. (2018). Pharmacometabolic response to pirfenidone in pulmonary fibrosis detected by MALDI-FTICR-MSI, European Respiratory Journal, DOI:10.1183/13993003.02314-2017. The combination of pharmacological and metabolomic methods ultimately led to an improved understanding of the disease mechanisms of idiopathic pulmonary fibrosis, as well as the mechanisms of action of pirfenidone.

PAXgene, an alcohol-based tissue fixative, enables comprehensive molecular tissues analysis

Urban, C. et al. (2017). PAXgene fixation enables comprehensive metabolomic and proteomic analyses of tissue specimens by MALDI MSI, Biochimica et Biophysica Acta, DOI:10.1016/j.bbagen.2017.10.005. An alcohol-based non-crosslinking tissue fixative, PAXgene Tissue System, has been successful tested as an alternative fixative to formalin for multi-omic tissue analysis.

MALDI imaging to access beta cells in their intact immediate environment

Aichler, M. et al. (2017). N-acyl taurines and acylcarnitines cause an imbalance in insulin synthesis and secretion provoking β cell dysfunction in type 2 diabetes, Cell Metabolism, DOI:10.1016/j.cmet.2017.04.012. While in type 1 diabetes the beta cells of the Langerhans islets in the pancreas are destroyed and absolute insulin deficiency occurs, type 2 diabetes is characterized […]